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Modes of Action of Exo-and Endo-β-1, 3-Glucanases from Irpex lacteus (Polyporus tulipiferae)
https://nagano.repo.nii.ac.jp/records/675
https://nagano.repo.nii.ac.jp/records/675fd9a6f1b-62d1-4ae0-9621-6511a8150d07
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
nagano_11-01-05.pdf (1.1 MB)
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| アイテムタイプ | Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2012-03-10 | |||||
| タイトル | ||||||
| タイトル | Modes of Action of Exo-and Endo-β-1, 3-Glucanases from Irpex lacteus (Polyporus tulipiferae) | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
WAKABAYASHI, Kazumasa
× WAKABAYASHI, Kazumasa |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | An experimental investigation was made on action patterns of two highly purified β-1, 3-glucanase components (named L-I and-IV), obtained from Driselase, a commercial enzyme preparation from Irpex lacteus (Polyporus tulipiferae), on various substrates. L-I and-IV exhibited a high substrate specificity in hydrolyzing β-1, 3-gluco-oligosaccharides with a degree of polymerization higher than 3, although their action patterns were different from each other, but they were inactive toward laminaribiose. L-I did not attack modified pachyman in which the nonreducing terminal residue had been altered by periodate oxidation, whereas L-IV attacked the modified pachyman at a rate of hydrolysis comparable with that of the original pachyman. These facts indicate that L-I cleaves the substrate molecule in an exo-wise manner, successively removing the glucosyl residue from the nonreducing terminal, whereas L-IV cleaves it in a random fashion. L-I can also bypass the β-1, 6-linked branches to cleave β-1, 3-linkages, producing glucose and gentio-biose when scleroglucan was used as substrate, but cannot cleave mixed-linkage β-1, 3-; β-1, 4-glucans such as lichenan and reduced, pneumo-coccal polysaccharide RS III. On the other hand, L-IV can cleave preferentially the β-1, 4-linkages adjacent to the β-1, 3-linked glucose residue in the mixed-linkage β-glucans, but scarcely have action on β-1, 3-glucans containing β-1, 6-linked branch. | |||||
| 書誌情報 |
長野大学紀要 en : ACADEMIC BULLETIN OF NACANO UNIVERSITY 巻 11, 号 1, p. 63-74, 発行日 1989-06-30 |
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| 出版者 | ||||||
| 出版者 | 長野大学 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0287-5438 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00179217 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
