@article{oai:nagano.repo.nii.ac.jp:00000517, author = {加藤, 宏一 and 佐藤, 英美}, issue = {1}, journal = {長野大学紀要, BULLETIN OF NAGANO UNIVERSITY}, month = {Jun}, note = {application/pdf, A marine dinoflagellate, Oxyrrhis marina was cultured in our laboratory being fed with E. coli. Morphological and biochemical characters of their nucleus and chromosomes were studied. In interphase nucleus, polytene chromosomes were observed attached to the nuclear pore by their ends. On mitosis, they moved in a cluster to the equatorial zone within the nucleus, but did not form a typical equatorial plate. Several bundles of intranuclear microtubules which connect two nuclear pores at two opposite poles were observed. The nuclear division proceed concomitant with the cytokinesis. Nuclei were isolated by a modified method for other dinoflagellates (Rizzo, '73). Nuclear fraction was then processed for extraction of histones by ordinary method. protein components of the extract were analyzed on the SDS-PAGE. In comparison with histones of a sea urchin extracted by the same method, it was found that Oxyrrhis lacks histones which universally coexists with chromatin of the eukaryote. In place of typical histones, a characteristic basic protein was detected. Biochemical characters, function(s) and spatial distribution of this protein were examined. Classification of several dinoflagellata was discussed on the basis of chromatin constitution.}, pages = {40--56}, title = {ハダカフタヒゲムシの染色体 : ヒストンに代わる核タンパク質の分離}, volume = {17}, year = {1995} }